Figure 1.

Dissection and dissociation of murine lung

(A) Schematic representation of crucial steps of murine lung dissection and dissociation to single cells suspension.

(B) Images illustrate murine lung before and after perfusion and inflation with digestion solution.

(C) Images illustrate steps after lung removal, separated lobes and lung mincing. Scale bar: 5 mm.

(D) Examples of lung dissociation at indicated incubation time with digestion solution.

(E) Visualization of cell pellet before and after red blood lysis buffer.