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Single-cell suspension and FACS sorting of murine AT2c
(A) Visualization of lung single-cell suspension.
(B) Gates for FACS sorting of AT2 cells.
(C) Validation of AT2s purity using cytospin preparations of sorted cells and staining with SFTPC (green). The yellow arrow indicates SFTPC negative cell. Scale bar:50 μm.
(D) Quantification of SFTPC+ in total DAPI+ cells. Data are presented as mean ± SEM. n=3.
