Figure 3.

Mincing and dissociation of human lung

(A) Visualization of human lung before and after pleura removal. Scale bar: 10 mm.

(B) Illustration of lung piece cut out for dissection. Blue circle depicted airway and vasculature that were dissected before lung mincing. Scale bar: 10 mm.

(C) Images taken at different steps of lung dissociation.

(D) Validation of human AT2s purity using cytospin preparations of HTII-280⁺ sorted cells and staining with HTII-280 (green) and SFTPC (red). The yellow arrow points out on SFTPC negative cell. Scale bar:50 μm.

(E) Quantification of SFTPC⁺ in total DAPI⁺ cells. Data are presented as mean ± SEM. n=3.