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Digestion solution for human lung
| Reagent | Final concentration | Amount |
|---|---|---|
| Collagenase type I (4500 units/mL) | 450 units/mL | 1.5 mL |
| Dispase I (50 units/mL) | 5 units/mL | 1.5 mL |
| DNase I (330 units/mL) | 10 units/mL | 454.5 μL |
| DMEM/F12 with 1× Anti-Anti | n/a | 11.545 mL |
| Total | n/a | 15 mL |
