REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit Polyclonal anti-Prosurfactant protein C (1:500)	Millipore	Cat # ab3786, RRID: AB_91588
Rat Monoclonal anti-RAGE/AGER (1:500)	R&D systems	Cat # MAB1179, RRID: AB_2289349
Goat Polyclonal anti-hRAGE/AGER (1:500)	R&D systems	Cat # AF1145, RRID: AB_354628
Mouse Monoclonal anti-HTII-280 (1:50)	Terrace Biotech	Cat # TB-27AHT2-280 RRID: AB 2832931
Rabbit Polyclonal anti-SFTPB (1:500)	Thermo Fisher Scientific	Cat # PA5-42000 RRID: AB_2609628
Alexa Fluor 488 goat anti-mouse IgM	Thermo Fisher Scientific	Cat # 10680 RRID: AB_10892893
Alexa Fluor 488 donkey anti-rabbit IgG	Thermo Fisher Scientific	Cat # A21206 RRID: AB_141708
Alexa Fluor 594 goat anti-mouse IgG1	Thermo Fisher Scientific	Cat # A21125, RRID: AB_2535767
Alexa Fluor 647 donkey anti-goat IgG	Thermo Fisher Scientific	Cat # A21447 RRID: AB_141844
Alexa Fluor 647 donkey anti-rat	Thermo Fisher Scientific	Cat # A48272 RRID: AB_2893138
Rat Monoclonal Brilliant Violet 711(TM) anti-mouse CD326 (EpCAM) (1:200)	BioLegend	Cat #118233 RRID: AB_2632775
Rat Monoclonal PE anti-mouse CD140a (1:200)	BioLegend	Cat # 135905 RRID: AB_1953268
Rat Monoclonal eFluor 450 anti-mouse CD31 (PECAM-1) (1:200)	Thermo Fisher Scientific	Cat # 48-0311-82 RRID: AB_10598807
Rat Monoclonal eFluor 450 anti-mouse CD45 (1:200)	Thermo Fisher Scientific	Cat # 48-0451-82 RRID: AB_1518806
Biological samples
Human lung tissue collected from males and females donors ranging from 1-70 years old	N/A	N/A
Chemicals, peptides, and recombinant proteins
SB431542	Abcam	Cat # Ab120163
CHIR99021	Tocris Bioscience	Cat # 4423
BIRB796	Tocris Bioscience	Cat # 5989
Heparin	Sigma-Aldrich	Cat # H3149
N-Acetyl-L-cysteine (NAC)	Sigma-Aldrich	Cat # A9165
hEGF	Gibco	Cat # PHG0313
Y27632 2HCl	Selleckchem	Cat # S1049
B-27 Supplement (50×)	Thermo Fisher Scientific	Cat # 17504044
N-2 Supplement (100×)	Thermo Fisher Scientific	Cat # 17502048
Insulin-Transferrin-Selenium (ITS) (100×)	Thermo Fisher Scientific	Cat # 41400-045
Glutamax	Thermo Fisher Scientific	Cat # 35050061
HEPES (1 M)	Thermo Fisher Scientific	Cat # 15630080
Antibiotic-Antimycotic (Anti-Anti) (100×)	Thermo Fisher Scientific	Cat # A5955-100ML
hFGF10	BioLegend	Cat # 559304
mIL-1β	BioLegend	Cat # 575104
hIL-1β	BioLegend	Cat # 579404
mNoggin	PeproTech	Cat # 250-38
mFGF10	R&D systems	Cat # 751004
Tamoxifen	Sigma-Aldrich	Cat # T5648
Dispase I	Corning	Cat # 354235
DNase I	Sigma-Aldrich	Cat # 10104159001
Collagenase type I	Gibco	Cat # 17100-017
Collagenase type I	Worthington	Cat # LS004197
Matrigel	Corning	Cat # 354230
Lysotracker (1:10000)	Thermo Fisher Scientific	Cat # L7526
Red Blood Cell Lysis Buffer	Sigma-Aldrich	Cat # 11814389001
Citrate Buffer, pH 6.0 (10×)	Sigma-Aldrich	Cat # C9999
Fluoromount-G, with DAPI	Thermo Fisher Scientific	Cat # 00-4959
PBS (1×)	Gibco	Cat # 20012027
Paraformaldehyde	Sigma-Aldrich	Cat # P6148
TrypLE™ Select Enzyme	Thermo Fisher Scientific	Cat # 12563029
O.C.T. Compound	Fisher Scientific	Cat # 23-730-571
Advanced DMEM/F-12	Thermo Fisher Scientific	Cat # 12634028
Trypan Blue Solution, 0.4%	Thermo Fisher Scientific	Cat # 15250061
ACK Lysing Buffer	Thermo Fisher Scientific	Cat # A1049201
Bovine Serum Albumin (BSA)	Sigma-Aldrich	Cat # A7906
Ethylenediaminetetraacetic acid disodium salt dihydrate (EDTA)	Sigma-Aldrich	Cat # E5134
Human TruStain FcX™ (Fc Receptor Blocking Solution)	BioLegend	Cat # 422302
Anti-Mouse IgM MicroBeads	Miltenyi Biotec	Cat # 130-047-302
Experimental models: Organisms/strains
C57BL/6J (males and females, 8–14 week old)	The Jackson Laboratory	Jax # 000664 RRID: IMSR_JAX:000664
Software and algorithms
FIJI	National Institutes of Health	https://fiji.sc
Other
Cell culture incubator	Thermo Fisher Scientific	Cat # 51033557
Heraeus Megafuge 8R Compact Centrifuge	Thermo Fisher Scientific	Cat # 75007213
Biosafety cabinet level 2 (tissue culture hood)	N/A	N/A
Hemocytometer	VWR	Cat # 470019-796
Incubated Tube Rotator - Hybaid Maxi 14 Rotisserie Hybridization Workstation	Thermo Fisher Scientific	Cat # 6246
Labquake™ Tube Shaker/Rotators	Fisher Scientific	Cat #13-687-12Q
Water Bath	Thermo Fisher Scientific	Cat # TSGP15D
Razor Blade (no.9)	VWR	Cat # 55411-050
Advanced Cell Strainers, 40 μm	Genesee Scientific	Cat # 25-375
Advanced Cell Strainers, 100 μm	Genesee Scientific	Cat # 25-375
Reducing Adapters for Cell Strainers	Genesee Scientific	Cat # 25-278
Tissue culture plate 24 well	SARSTEDT INC	Cat # 83.3922
Tissue culture plate 6 well	SARSTEDT INC	Cat # 83.3920
Syringe filter, PES, 0.22 μm	Genesee Scientific	Cat #25-243
BD 10 mL Syringe	BD	Cat # 302995
3-0 Silk Suture Spool, Black Braid	LOOK	MFIDSP117
22G needle	BD	Cat # 305155
15 mL Conical Sterile Polypropylene Centrifuge Tubes	Thermo Fisher Scientific	Cat # 339650
50 mL Conical Sterile Polypropylene Centrifuge Tubes	Thermo Fisher Scientific	Cat # 339652
MACS MultiStand	Miltenyi Biotec	Cat # 130-042-303
MidiMACS Separator	Miltenyi Biotec	Cat # 130-042-302
LS Column	Miltenyi Biotec	Cat # 130-042-401
Cell Lifter, Double End Flat Blade & J-Hock	CELLTREAT Scientific Products	Cat # 229306
Falcon® 5 mL Round Bottom Polystyrene Test Tube, with Cell Strainer Snap Cap	Corning	Cat # 352235
500 mL vacuum Filter/Storage System, PES, 0.22 μm	Corning	Cat # 431097
250 mL vacuum Filter/Storage System, PES, 0.22 μm	Corning	Cat # 431096
Cryomold 10 mm × 10 mm × 5 mm	VWR	Cat # 25608-922
Cryomold 15 mm × 15 mm × 5 mm	VWR	Cat # 25608-924
1.7 mL tubes	Genesee Scientific	Cat # 24-282