. 2022 May 13;101(8):101962. doi: 10.1016/j.psj.2022.101962

Table 2.

Gene-specific primers sequences for quantitative real-time PCR.

Gene name¹	GenBank²	Primer sequence³ (5′→3′)	Length
IL-6	AB302327.1	AACAACCTCAACCTGCCCAA	112
IL-6	AB302327.1	AGGTCTGAAAGGCGAACAGG	112
IL-8	DQ393272.2	CCTCCTCCTGGTTTCAGCTG	136
IL-8	DQ393272.2	TGGCGTCAGCTTCACATCTT	136
IL-10	NM_012854.2	CAGACCAGCACCAGTCATCA	96
IL-10	NM_012854.2	TCCCGTTCTCATCCATCTTCTC	96
NF-κB	NM_001012887.2	AAGATCTGGTGGTGTGCCTG	137
NF-κB	NM_001012887.2	AGTGGAACCTTTCGCGGATT	137
IκBα	NM_001001472.2	CAGCACTACACTTGGCCGTA	101
IκBα	NM_001001472.2	GGAGTAGCCCTGGTAGGTCA	101
TNF-α	HQ739087.1	GAACCCTCCGCAGTACTCAG	116
TNF-α	HQ739087.1	AACTCATCTGAACTGGGCGG	116
TLR4	KP410249.1	CGGCTCCGCATCTTGGATAT	148
TLR4	KP410249.1	GGGCTTGGAGTGGCTTGTAT	148
IFN-γ	NM_205149.1	TGTAGCTGACGGTGGACCTA	134
IFN-γ	NM_205149.1	GCGGCTTTGACTTGTCAGTG	134
β-Actin	NM_205518.1	ACCGGACTGTTACCAACACC	116
β-Actin	NM_205518.1	CCTGAGTCAAGCGCCAAAAG	116

Abbreviations: IL, interleukin; IκBα, inhibitor of NF-κB alpha; IFN-γ, interferon-γ; TNF-α, tumor necrosis factor-α; TLR4, toll-like receptor 4.

GenBank Accession Number.

Shown as the forward primer then the reverse primer.