TABLE 2.

Probes for hybridization experiments and the positions of primers that were used to generate the probes by PCR

Probe	Primer designations or locations	Restriction site within the probe
M. trichosporium OB3b
pmoA	A189 and A682a	SalI 1683, BclI 1954
OB1	111–128, 478–461	BamHI 255
OB2	656–674, 1042–1023	SalI 773
OB3	2162–2180, 2567–2549	BglII 2266
Methylocystis sp. strain M
pmoA	A189 and A682a	SalI 2132, BclI 2402
pmoC	C126 (126–145) and C572 (572–551)b	EcoRI 1046, SalI 1150
pC1	302–319, 651–634	HpaI 395
pC59	1169–1185, 1589–1572	SalI 1146, BclI 1447, PstI 1578
p286	2016–2033, 2418–2435	SalI 2127, BclI 2398

^aSee reference 13. 

^bBased on pmo copy 1 (accession number L40804) from M. capsulatus Bath.