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. 2000 Mar;66(3):966–975. doi: 10.1128/aem.66.3.966-975.2000

TABLE 4.

Hybridization experiments showing the origins of the cloned regions of the pmo gene cluster for M. trichosporium OB3b

Fragment Size(s) of band(s) (kb)a with probeb:
OB1
OB2
OB3
Observed Expected Observed Expected Observed Expected
BamHI (1.8), 2.7, (>10.0) >0.155, 2.714 2.7 2.714 2.7, (4.2) 2.714
BglII (1.9), 5.2 >0.527 1.7, (3.5) 1.639 1.7, (7.5, >9.0) 1.639, 2.282
SalI 0.95, 1.65 >0.673 0.7, (0.95, 1.65, 1.7) 0.689, (>0.6) (0.7), 1.8 1.841
SphI 6.5 >2.496 6.5 >2.964 6.5 >2.496
a

Observed, after hybridization; Expected, calculated according to the restriction pattern (Fig. 2 and 3). Sizes given in parentheses indicate weak bands. 

b

See Table 1 and Fig. 2 and 3 for positions of probes.