TABLE 5.

Hybridization experiments showing the origins of the cloned regions of the pmo gene cluster for Methylocystis sp. strain M

Fragment	Size(s) of band(s) (kb)a with probeb:
	pC1		pC59		p286
	Observed	Expected	Observed	Expected	Observed	Expected
BamHI	6.0	>3.425	6.0, >10.0	>3.425	6.0, (>10.0)	>3.425
BglII	7.5, (>10.0)	>2.704	7.5, (>10.0)	>2.704	7.5, (>10.0)	>2.704
PstI	1.7, (>10.0)	1.572	1.7, (2.5, >10.0)	>1.572	3.5, 4.5	3.468
XhoI	2.8, 2.9	>1.927	2.4, 2.8, 2.9	>1.927	1.4	1.431

^aObserved, after hybridization; Expected, calculated according to the restriction pattern (Fig. 2 and 3). Sizes given in parentheses indicate weak bands. 

^bSee Table 1 and Fig. 2 and 3 for positions of probes.