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. 2022 May 31;9:898674. doi: 10.3389/fnut.2022.898674

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Copyright © 2022 Miao, Yu, Li, Sun and Guo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scheme for elucidation of polysaccharide structure based on degradation. Partial acid hydrolysis with low concentrations of TFA is generally used to remove degradable side chains of polysaccharides. Enzymatic hydrolysis with glycosidase is a more specific method for degradation of glycosyls. The released oligosaccharides and the degradation-resistant fraction can be separated by dialysis using dialysis bags with Mw cutoff < 5.0 kDa or size-exclusion columns such as Bio-Gel P4 or P6. The obtained products can be further analyzed by mass spectrometry, methylation analysis, and NMR experiments. TFA, trifluoroacetic acid; NMR, nuclear magnetic resonance.