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. 2022 Jun 9;3:128–135. doi: 10.1016/j.crimmu.2022.05.005

Fig. 1.

Fig. 1

Analysis of intact CD16a V158. A. Gel filtration of CD16a using a Superdex 75 column following TEV digestion. B. SDS-PAGE analysis of the central peak. C. SDS-PAGE analysis of select CD16a fractions before and after treatment with EndoF. D. FT-ICR MS analysis of EndoF-treated CD16a fractions. The observed masses are reported in Table 2. GFP form 1 includes residues KIEW through the TEV cleavage site, and GFP form 2 starts with DIFEA and ends with the TEV cleavage site. “XX+” indicates charge state.