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N-glycan occupancy for the N74 site of CD16a V158 as observed with two peptides in [16O]-water. Recorded spectra corresponding to each peptide are shown in the top row. The calculated isotope distributions for Asn- and Asp-containing peptides are shown in the second and third rows, respectively. The final row shows the fitted Asn + Asp data to determine the percentage of peptides that showed N-glycosylation.
