TABLE 3.
Distribution of 14C-residues after incubation of PAH-degrading isolates with [14C]benzo[a]pyrene in soil cultures
| Soil and microorganism | % Recovery of added radiolabel from various culture fractionsa
|
||
|---|---|---|---|
| 14CO2 | DCM phase | Total recovery | |
| PAH-spiked soil | |||
| VUN 10,009b | 12.3 | 84.8 | 97.1 |
| VUN 10,010b | 24.1 | 73.9 | 98.0 |
| VUO 10,201b | NA | 97.7 | 97.7 |
| Coculture A | 37.7 | 61.4 | 99.1 |
| Coculture B | 44.7 | 53.6 | 98.3 |
| PAH-contaminated soil from Sydney | |||
| Indigenousc | 4.8 | 93.1 | 97.9 |
| VUN 10,009b | 18.8 | 80.6 | 99.4 |
| VUN 10,010b | 31.5 | 67.3 | 98.8 |
| VUO 10,201b | 8.7 | 89.1 | 97.8 |
| Coculture A | 37.7 | 60.8 | 98.5 |
| Coculture B | 53.2 | 44.2 | 97.4 |
a
Mean values of percentage of recovery for duplicate 100-day cultures. The standard deviation for recovery of added label was less than 5%. The amount of radiolabel in aqueous extracts and biomass fractions was not determined. NA, not appreciable.
b
Axenic inocula were used.
c
Uninoculated soil.