TABLE 4.
Mutagenicity of DCM extracts from PAH-contaminated liquid and soil cultures
| Culture from which DCM extracts were obtained | No. of revertants/platea
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Benzo[a]pyreneb
|
Dibenz[a,h]anthraceneb
|
PAH-spiked soilc
|
PAH-contaminated soilc
|
|||||||||
| Day 0 | Day 28 | Day 56 | Day 0 | Day 28 | Day 56 | Day 0 | Day 56 | Day 100 | Day 0 | Day 56 | Day 100 | |
| Uninoculated control | 687 ± 6 | 704 ± 8 | 701 ± 6 | 444 ± 5 | 438 ± 6 | 447 ± 7 | 689 ± 11 | 671 ± 14 | 693 ± 6 | 718 ± 4 | 711 ± 8 | 689 ± 4 |
| VUN 10,009d | 696 ± 8 | 688 ± 9 | 657 ± 6 | 437 ± 7 | 411 ± 6 | 388 ± 1 | 702 ± 14 | 686 ± 9 | 594 ± 7 | 724 ± 9 | 703 ± 11 | 677 ± 12 |
| VUN 10,010d | 682 ± 3 | 651 ± 8 | 632 ± 13 | 441 ± 4 | 407 ± 5 | 384 ± 2 | 695 ± 7 | 623 ± 10 | 501 ± 16 | 722 ± 20 | 697 ± 6 | 648 ± 4 |
| VUO 10,201d | 673 ± 10 | 337 ± 13 | 339 ± 4 | 452 ± 7 | 392 ± 3 | 382 ± 8 | 697 ± 5 | 477 ± 12 | 367 ± 8 | 716 ± 7 | 634 ± 3 | 461 ± 8 |
| Coculture A | 682 ± 6 | 306 ± 11 | 272 ± 7 | 449 ± 10 | 296 ± 8 | 291 ± 4 | 705 ± 3 | 392 ± 10 | 295 ± 11 | 722 ± 8 | 617 ± 13 | 420 ± 6 |
| Coculture B | 687 ± 14 | 294 ± 5 | 253 ± 9 | 460 ± 16 | 277 ± 10 | 276 ± 3 | 698 ± 7 | 367 ± 15 | 263 ± 6 | 719 ± 4 | 576 ± 6 | 409 ± 4 |
a
Assay was performed using serovar Typhimurium TA100 with the postmitochondrial activation system (S9). DCM extracts were exchanged into DMSO: the number of revertants per plate for the DMSO control was 124 ± 3.
b
Mutagenicity assay on DCM extracts of BSM containing either benzo[a]pyrene or dibenz[a,h]anthracene as a sole carbon and energy source.
c
Mutagenicity assay on DCM extracts of PAH-spiked soil cultures and PAH-contaminated soil cultures.
d
Axenic inocula were used.