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. 2022 Jun 6;13:38–46. doi: 10.1016/j.ibneur.2022.06.001

Fig. 2.

Fig. 2

Effects of Short-Term Food Deprivation (FD) on Dopamine-Beta-Hydroxylase (DβH) and TH Gene Expression in A2 Noradrenergic Neurons Innervating the rPO of Estradiol-Implanted OVX Female Rats. Graphs depict mean normalized DβH mRNA levels +/- S.E.M. measured in cytoplasmic epifluorescence-positive, cytoplasmic TH-ir-positive, nGKRP-ir-positive A2 neurons (Fig. 2A) or cytoplasmic epifluorescence-positive, cytoplasmic TH-ir-positive, nGKRP-ir-negative (Fig. 2B) A2 neurons collected from FF/E-300 (solid white bars; n = 4 nGKRP-ir-positive A2 nerve cells; n = 4 nGKRP-ir-negative A2 neurons), FD/E-300 (striped white bars; n = 4 nGKRP-ir-positive A2 nerve cells; n = 4 nGKRP-ir-negative A2 neurons), FF/E-30 (solid gray bars; n = 4 nGKRP-ir-positive A2 nerve cells; n = 4 nGKRP-ir-negative A2 neurons), or FD/E-30 (striped gray bars; n = 4 nGKRP-ir-positive A2 nerve cells; n = 4 nGKRP-ir-negative A2 neurons) treatment groups. Data were analyzed by two-way ANOVA and Student-Neuman-Keuls post-hoc test, using GraphPad Prism (Volume 8) software; statistical results are presented in Supplementary Table 1. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.