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. 2022 May 30;25(6):104491. doi: 10.1016/j.isci.2022.104491

Figure 3.

Figure 3

To characterize optimal targeting sites of PaeCascade-mediated CRISPRi in S. oneidensis

(A) Targeting sites of designed crRNAs binding different regions of gfp in the genome of the strain S. oneidensis GZ by type I-F PaeCascade-mediated system.

(B) The inhibition efficiency of type I-F PaeCascade system targeting different regions of gfp. The values above the bar indicate the corresponding repression folds. Median GFP levels are normalized to the control strain cr0-GZ. Values and error bars indicate mean ± SEM of three replicates.