Figure 6.

PaeCascade-RpoD system also successfully enabled transcriptional inhibition in S. oneidensis MR-1

(A) The schematic diagram of gene repression by PaeCascade-RpoD.

(B) Transcriptional inhibition was enabled by PaeCascade-RpoD targeting gfp. Targeting sites of designed crRNAs of gfp in the plasmid pTarget and the inhibition efficiency of type I-F PaeCascade-RpoD system. Median GFP levels are normalized to the control strain cr0. Values and error bars indicate mean ± SEM of three replicates.