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. 2022 May 30;25(6):104491. doi: 10.1016/j.isci.2022.104491

Figure 8.

Figure 8

Simultaneous activation and inhibition by PaeCascade-RpoD in S. oneidensis MR-1

(A) Plasmid map of pTargetGB for simultaneous regulation.

(B) Plasmid map of pPaeR containing two crRNA expression cassettes for activating GFP and inhibiting BFP.

(C) Schematic diagram and simultaneous regulation efficiency of PaeCascade-RpoD. GFP (a) indicates GFP activation, and BFP (i) indicates BFP inhibition. “+” stands for performing corresponding regulation, and “-” stands for without corresponding regulation. “-88” or “-171” means the targeting position for activating GFP. Median fluorescence is normalized to the non-target strain. Values and error bars indicate mean ± SEM of three replicates.