FIG. 1.
Effect of chlorhexidine solution (50 μg/ml) on trophozoites (A) and cysts (B) on A. polyphaga (2 × 106 cells/ml) stained with PI. FL2-H versus FSC-H dot plots were used to discriminate viable (PI excluded; lower quadrant) from dead (PI stained; upper quadrant) cells. The dot plots were gated by forward scatter to eliminate analysis of noise and smaller particles (<3 μm in diameter). Analysis gates were set with fluorescence from control live (FL2-H, ≤540) and heat-killed (FL2-H, >540) amoebae.