TABLE 1.
Loss of viability of A. castellanii and A. polyphaga in disinfectant solutions containing chlorhexidine and PHMB as determined by PI staining and methylene blue exclusion
| Amoebaea | % Reduction byb:
|
|||||
|---|---|---|---|---|---|---|
| Flow cytometry
|
Methylene blue exclusion
|
|||||
| CH | PHMB | PBS | CH | PHMB | PBS | |
| A. castellanii | ||||||
| Trophozoitesc | 93–99 | 13–24 | 8–17 | 94–99 | 25–68 | 8–12 |
| Cystsc | 87–94 | 17–29 | 8–10 | 91–99 | 18–20 | 16–21 |
| A. polyphaga | ||||||
| Trophpozoites | 89–100 | 6–17 | 3–13 | 96–99 | 22–81 | 10–16 |
| Cysts | 73–94 | 14–19 | 4–10 | 92–97 | 17–21 | 5–9 |
a
Inocula of 105 to 106 amoebae/ml (n = 6).
b
CH, chlorhexidine (50 μg/ml). PHMB was used at 0.5 μg/ml.
c
Data normalized for loss of viability in PBS.