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. 2022 May 28;62:101518. doi: 10.1016/j.molmet.2022.101518

Figure 1.

Figure 1

Psmb4 controls adipogenesis and adipocyte health. (A) Relative gene expression of Psmb4 at different time points after knockdown with scrambled or Psmb4 siRNA. (B,C) Representative immunoblot of Psmb4 in siScrambled and siPsmb4 adipocytes with (C) protein quantification normalized to β-tubulin. (D) Relative gene expression of adipogenesis and stress markers adipocytes after Psmb4 knockdown measured on day 3 of differentiation. (E) Relative gene expression of adipogenesis and stress markers adipocytes after Psmb4 knockdown measured on day 5 of differentiation. (F) Representative Oil-Red-O staining and quantification (day 5). (G) Relative free glycerol levels in adipocyte supernatant (day 5) after DMSO or 1 μM CL316,243 treatment for 3 h. Data are mean ± SEM. Unless otherwise specified: n = 6 independent measurements from 2 separate experiments. Significant if P < 0.05, indicated by (∗) or different letters.