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. 2022 May 28;62:101518. doi: 10.1016/j.molmet.2022.101518

Figure 3.

Figure 3

Loss of Psmb4 initiates a proteostatic stress-response via Nfe2l1. (A) Relative gene expression of Psmb4 and Nfe2l1 at different time points after knockdown with scrambled, Psmb4 and/or Nfe2l1 siRNA. (B,C) Representative immunoblots of Nfe2l1, Psmb4, Psmd2 and β-tubulin in siScrambled, siPsmb4, siNfe2l1 or siPsmb4 + siNfe2l1 adipocytes (day 5) with (C) protein quantification levels normalized to β-tubulin.(D) Relative gene expression of various proteasome subunits. (E) Trypsin-like, chymotrypsin-like, and caspase-like proteasome activity in adipocytes (day 5), normalized to DNA content. (F) Relative gene expression of adipogenesis and stress markers in adipocytes (day 5). (G) Relative gene expression of inflammation markers in adipocytes (day 5). (H) Relative gene expression of ER stress and unfolded protein response markers in adipocytes (day 5). Data are mean ± SEM. Unless otherwise specified: n = 6 independent measurements from 2 separate experiments. Significant if P < 0.05, indicated by (∗) or different letters.