Loss of Psmb4 induces inflammation and blocks adipogenesis via Atf3. (A) Relative gene expression of Psmb4, Nfe2l1, and Atf3. (B) Viability in adipocytes. (C) Trypsin-like, chymotrypsin-like, and caspase-like proteasome activity in adipocytes (day 5), normalized to DNA content (D) Relative gene expression of adipogenesis and stress markers in adipocytes (day 5) after knockdown. (E) Oil-Red-O staining in adipocytes after knockdown. (F) Supernatant free glycerol levels after treatment with DMSO or 1 μM Norepinephrine (NE) for 1 h normalized to protein. (G) Relative gene expression of lipases in adipocytes (day 5). (H, I) Oxygen consumption rate (OCR) in adipocytes after knockdown, normalized to DNA levels (n = 8, from 2 experiments). Unless otherwise specified: n = 6 independent measurements from 2 separate experiments. Data are mean ± SEM. Significant if P < 0.05, indicated by (∗) or different letters.