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. 2022 Jun 10;15(6):dmm049517. doi: 10.1242/dmm.049517

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 3. — Wholemount in situ hybridization results on Wnt genes and additionally relevant Wnt signaling downstream target genes around the PNP regions of littermate controls and Pax3-Cre;Lrp6-cKOs at E9.5. (A,B) Msx1 is restrictively expressed in the dorsal PNP of the normal control embryo (bracket in A), and its expression is significantly diminished in the mutant PNP (bracket in B). (C-J) No obvious changes in T (C,D), Tbx6 (E,F), Wnt1 (G,H) and Wnt5a (I,J) expression patterns around the mutant PNP regions were observed compared to respective expression patterns in the littermate controls.