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. 2022 Jun 10;15(6):dmm049517. doi: 10.1242/dmm.049517

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© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 5. — Noncanonical Wnt/PCP signaling activities in littermate controls and Pax3-Cre;Lrp6-cKOs at E9.5. (A-D) Transverse sections after wholemount in situ hybridization show no obvious changes in Vangl2 (A,B) and Ptk7 (C,D) expression patterns at the PNP closure sites in normal control and mutant embryos. (E) Immunoblots show no differences in phosphorylated (higher band that is linked with PCP signaling) and nonphosphorylated (lower band) Dvl2 proteins between the control and mutant PNP samples.