TABLE 3.
Dissociation constants of tubulin—benzimidazoles-, podophyllotoxin-, and combretastatin A4 - interaction measured by competition titration with MDL.
| Drug | Brain tubulin (BTb) | Erythrocyte tubulin (CeTb) | ||
|---|---|---|---|---|
| IC50, µM | Kd, µM | IC50, µM | Kd, µM | |
| Nocodazole | 11 ± 1.2 | 1.0 ± 0.11 | 1.5 ± 0.11 | 0.51 ± 0.036 |
| Mebendazole | 14 ± 2.5 | 1.3 ± 0.23 | 6.8 ± 0.73 | 2.3 ± 0.25 |
| Carbendazim | nb | nb | 37 ± 5.4 | 13 ± 1.8 |
| Oxibendazole | nb | nb | 36 ± 5.6 | 12 ± 1.9 |
| Albendazole | nb | nb | 104 ± 12 | 35 ± 4.0 |
| Podophyllotoxin | 4.0 ± 1.0 | 0.37 ± 0.035 | 15 ± 0.53 | 4.9 ± 0.18 |
| Combretastatin A4 | 2.6 ± 0.22 | 0.13 ± 0.011 | 20 ± 1.2 | 5.7 ± 0.32 |
Kd was calculated with Equation 4.
Uncertainties are the standard errors of the fits. nb, no binding detected.