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. 2022 May 31;10:890389. doi: 10.3389/fcell.2022.890389

FIGURE 2.

FIGURE 2

RSV infection of hAECs induces the biphasic release of HMGB1. hAECs were inoculated with RSV (MOI of 1) and the cells analysed at 0, 2, 6, 12, 18, 24, and 48 h post infection (hpi). (A) Quantification and representative images (magnification ×20) of nuclear- and cytoplasmic-HMGB1 positive hAECs. (B) HMGB1 levels in the cell culture supernatant. (C) HMGB1 gene expression normalized to the house-keeping gene. (D) Quantification of RSV positive hAECs. (E) Number of viral copies (expression of RSV N gene) normalized to a house-keeping gene. Each data point represents 4-6 donors. The data are presented as the mean ± SEM. The data were analyzed using a two-way ANOVA and Sidak’s multiple comparisons test. *p < 0.05, **p < 0.01, ***p < 0.005, denotes significance between infected and uninfected cells at the specified time point.