TABLE 3.
Effect of in ovo inclusion of Bacillus subtilis, raffinose, and their synbiotics on growth performance of 21-day-old broilers.
| Items | Treatment groups1 |
SEM | P-value | |||||||||
| NC | PC | BS1 | BS2 | R1 | R2 | BS1R1 | BS1R2 | BS2R1 | BS2R2 | |||
| Body weight, g | ||||||||||||
| 21 d | 909.67e | 926.75e | 960.67cd | 977.00bc | 946.46d | 962.47cd | 990.97b | 1012.61a | 977.90bc | 977.63bc | 5.60 | <0.001 |
| Feed intake, g | ||||||||||||
| 0-21 d | 1085.99f | 1090.33ef | 1116.97cde | 1123.88bcd | 1102.40de | 1124.67bcd | 1150.23ab | 1159.36a | 1127.43bcd | 1138.06abc | 4.87 | <0.001 |
| Feed conversion ratio | ||||||||||||
| 0-21 d | 1.19a | 1.18b | 1.16cd | 1.15cde | 1.16cd | 1.17bc | 1.16cd | 1.14e | 1.15cde | 1.16cd | 0.003 | <0.001 |
Means in a row with different superscripts are significantly different (p < 0.05).
1NC, non-injected group; PC, sterile distilled water; BS1 = Bacillus subtilis 4 × 105/egg; BS2 = Bacillus subtilis 4 × 106/egg; R1 = Raffinose 2 mg/egg; R2 = Raffinose 3 mg/egg; BS1R1 = (Bacillus subtilis 4 × 105 + Raffinose 2 mg)/egg; BS1R2 = (Bacillus subtilis 4 × 105 + Raffinose 3 mg)/egg; BS2R1 = (Bacillus subtilis 4 × 106 + Raffinose 2 mg)/egg; BS2R2 = (Bacillus subtilis 4 × 106 + Raffinose 3 mg)/egg. SEM, standard error of means.