Table 1.
Gene-specific primers used for quantitative real-time PCR analysis.
| Gene | Accession | Primer sequences (5'–3') | Product size (bp) |
|---|---|---|---|
| CLDN1 | XM_005513213.2 | TCTTTGGTGGGGTGATGT | 109 |
| TTGACAGGGGTGTAAGGG | |||
| CLDN2 | XM_021283269.1 | GTGCAGATGGGAACAAGGT | 119 |
| GAGCCAAGGAAGCTACGG | |||
| CLDN3 | XM_005515008.2 | ACCTCATCCCCGTCTCCT | 109 |
| CAGCCCACGTAGAGCGA | |||
| CLDN4 | XM_021282730 | AATGAAGCTGTTGGAAGGG | 135 |
| ATCTGGGTTCAAAGATGGC | |||
| CLDN16 | XM_005513212.2 | ACAAAATGCCGTGGACTGTG | 134 |
| GCTGTGATCATCATGGCTCG | |||
| IL1-β | NM_001282824.1 | CTGGACTTGGAAGCGACA | 102 |
| GGCCACCATGAGGACAG | |||
| IL4 | XM_005512458 | CTGTGACAAGATGAACGCGA | 123 |
| ATTCCTTCGAGGTGGTTGTGG | |||
| IL6 | XM_013369893.2 | GTGCAAAGCTCAAGTCCAA | 108 |
| AATAGCGAACAGCCCTCAC | |||
| IL10 | XM_021298374.1 | GACCAGCACCCTTCACC | 111 |
| TTGCTCCTCTTCTCGCA | |||
| IL12 | XM_021288847.1 | GAGTGCCAGAAAGAAAAC | 154 |
| CATACTGACATTGGGGTG | |||
| MUC2 | XM_021283472.1 | GAATGTGCCAAACAAAGC | 231 |
| CTGATGTACGCAAACCCT | |||
| MUC5AC | XM_021299062.1 | AAGACCTTTGACGGAGAC | 136 |
| GGTTGATTGTTGGAGCAT | |||
| MUC13 | XM_005505089.3 | TGGGATGTTCTTTCTTGT | 246 |
| AGTGACTGCTATGGCTAA | |||
| OCLN | XM_005509325.2 | CAGGACGTGGCAGAGGA | 105 |
| GTGGAAGAGCTTGTTGCGT | |||
| PIGR | XM_005510390.3 | GAGGGCTCTCCCACAAAGTC | 136 |
| CTCACGCTCTCGTTGTCCTT | |||
| TJP1 | XM_021299314.1 | GAACCAAAGCCAGTGTATG | 159 |
| GGTCCCCTTCCTCTAATC | |||
| TJP2 | XM_021283254.1 | CGTCCTCGGTCGTGTTCATC | 228 |
| TGTAGTGAAACGACCAAGGAAGG | |||
| TLR2t1 | XM_021285395 | GCTGGGACCCTCTTTCTTGG | 156 |
| TCCTGAAAGCAGAGCCTTGG | |||
| TLR2t2 | XM_013372151.2 | CTACCTGGAGTGGCCTCCT | 137 |
| ACATGCAAGGCAAACAAATCC | |||
| TLR3 | XM_005500210.2 | GGAAATCTCTTTGGCCTTG | 91 |
| GAATCGCCGTGTTTGATAA | |||
| TLR4 | XM_005498384.2 | TGTTTACAGCCACCCAGTT | 145 |
| GGACTCAGCCTCATTGCT | |||
| TLR5 | KM086723.1 | CAGCTTGTGTGGGAGAGAGG | 103 |
| CCTGTGGAAGAGCACTGAGG | |||
| TLR7 | XM_005512700.2 | CAATCAGTCCCAAAGCTCA | 79 |
| TAAACATCTGTGCTGGCAA | |||
| TNF-α | XM_005506079.2 | GTGTTCAGTCCCTCCTCGTT | 230 |
| TCAATCACAAGCAATGGGAGC | |||
| GAPDH | NM_001282835.1 | CCGGGCTGCTATTCTCTCTG | 141 |
| TTCCCATTCTCGGCCTTGAC |
CLDN, claudin; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MUC, mucin; OCLN, occludin; PIGR, polymeric immunoglobulin receptor; TJP, tight junction protein; TLR, toll-like receptor; TNF, tumor necrosis factor.