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. 2022 May 31;12:858979. doi: 10.3389/fcimb.2022.858979

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Copyright © 2022 Ferreira, Mendoza, Gonçalves, Rodríguez-de la Noval, Honorato, Nimrichter, Ramos, Nogueira, Domont, Peralta and Guimarães

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Acanthamoeba castellanii and macrophage extracts showed a dose-dependent binding to mannose and mannan. Indirect ELISA demonstrated dose dependent binding of clarified extracts of (A) A. castellanii (Ac) and (B) RAW 264.7 macrophages to mannan and mannose. (C) MPPs of Ac and macrophages displayed a more intense binding to mannose than the phagocyte’s biotinylated surface extracts. In addition, MPPs of macrophages seem to bind more effectively to mannose in comparison to Ac MPPs. The streptavidin-alkaline phosphatase conjugate was used to detect biotinylated proteins. As a negative control, clarified non-biotinylated extracts were used in the process and displayed no reactivity.