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. 2022 May 31;12:858979. doi: 10.3389/fcimb.2022.858979

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Copyright © 2022 Ferreira, Mendoza, Gonçalves, Rodríguez-de la Noval, Honorato, Nimrichter, Ramos, Nogueira, Domont, Peralta and Guimarães

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mannose-purified proteins (MPPs) of Acanthamoeba castellanii and macrophages recognized targets on the fungal cell wall. (A–C) Histograms representing the FL1-H fluorescence and binding intensity of phagocytes MPPs to fungi (A) Candida albicans (Ca), (B) Cryptococcus neoformans (Cn) and (C) Histoplasma capsulatum (Hc), with the respective co-incubation with Concanavalin A (ConA) for inhibition evaluations. (D–F) Besides, yeast labeling with the positive control ConA data on (D) Ca, (E) Cn and (F) Hc, which were analyzed by FL2-H intensity, are demonstrated, along with respective incubations with the MPPs of each phagocyte for inhibition evaluation.