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. 2022 May 31;12:858979. doi: 10.3389/fcimb.2022.858979

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Copyright © 2022 Ferreira, Mendoza, Gonçalves, Rodríguez-de la Noval, Honorato, Nimrichter, Ramos, Nogueira, Domont, Peralta and Guimarães

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mannose purified proteins (MPPs) of Acanthamoeba castellanii and RAW 246.7 macrophages co-localized with a mannose-specific lectin Concanavalin A. Fluorescence microscopy images demonstrate the cell wall of the fungi (A) Candida albicans; (B) Cryptococcus neoformans and (C) Histoplasma capsulatum stained in blue (Uvitex 2B); MPPs of phagocytes stained in green upon incubations with yeasts, followed by the streptavidin-Alexa 488 conjugate; and the mannosylated components of the yeast cell wall stained in red, after incubations with ConA-TRITC. Scale bar= 5μm.