Figure 7.

The mannose receptor plays an important role in the events of fungal interaction with Acanthamoeba castellanii and macrophages. After NHS-Rhodamine labeling of (A) Candida albicans, (B) Cryptococcus neoformans and (C) Histoplasma capsulatum, interaction assays were performed with both A. castellanii and RAW 264.7, with phagocytes model indicated on the graphs, in two conditions: absence (Control group, black bars) and presence of 100 mM soluble mannose (Mannose 100 mM, white bars) and analyzed upon 30 min and 2 h. The interaction ratios were determined in each case, and inhibition percentages (numbers above bars) calculated as followed: (Control - Pre-treated group) x 100/Control. The statistical significances comparing inhibition x controls are displayed above each group (**p < 0.01; ***p < 0.001; ****p < 0.0001; ns, not significant). The bars represent the average of three independent experiments, performed in triplicates.