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. 2022 May 31;16:905783. doi: 10.3389/fnbeh.2022.905783

Figure 4.

Figure 4

HeterozygousKdm6b knockout mice have reduced anxiety-like behaviors under stress. (A,B) The plots showing the number of male (A) and female (B) wild-type and heterozygous Kdm6b-KO mice entering the arena center in a5-min period for 30 min in the open-field chamber. P-value calculated using two-way ANOVA test. The results shown are the effect of genotype on the number of mice entering the arena center (male: F1, 84 = 21.07, p < 0.0001, female: F1, 72 = 4.89, p = 0.03). Error bars in graphs represent mean ± SEM. (C) Representative traces of wild-type and heterozygote Kdm6b-KO mice in the marble burying testing cages. (D,E) The bars showing the running distances of male (D) and female (E) wild-type and heterozygous Kdm6b-KO mice in the marble burying testing cages. n = 8 males and 7 females per genotype. P-values calculated using a two-tailed t-test. *p < 0.01; ns: not significant. Error bars in graphs represent mean ± SEM. (F) Representative photos showing the unburied marbles after marble burying tasks. (G,H) The bars showing the numbers of full and partial buried marbles by male (G) and female (H) wild-type and heterozygous Kdm6b-KO mice in the marble burying test. n = 8 males and 7 females per genotype. P-value calculated using two-way ANOVA test and Sidak’s multiple comparisons test. The results shown are the effect of genotype on the number of buried marbles (male:F1, 28 = 8.52, p = 0.0069, female:F1, 24 = 3.88, p = 0.04). Error bars in graphs represent mean ± SEM. (I) Representative traces of male wild-type and heterozygous Kdm6b-KO mice in the open and closed arms of elevated plus maze. (J,K) The bars showing the number of male (J) and female (K) wild-type and heterozygous Kdm6b-KO mice entering the open arms in the elevated plus maze. n = 8 males and 7 females per genotype. P-values calculated using a two-tailed t-test. ***p < 0.001; ns: not significant. Error bars in graphs represent mean ± SEM. (L,M) The bars showing the running time of male (L) and female (M) wild-type and heterozygous Kdm6b-KO mice in the open arms of the elevated plus maze. n = 8 males and 7 females per genotype. P-values calculated using a two-tailed t-test. Note: ***p < 0.001, ns: not significant. Error bars in graphs represent mean ± SEM.