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. 2022 May 31;16:905783. doi: 10.3389/fnbeh.2022.905783

Figure 6.

Figure 6

Methylphenidateameliorates both locomotor hyperactivity and impulsivity inheterozygous Kdm6b knockout mice. (A,B) The plotsshowing the running distances of male (A) and female(B) wild-type and heterozygous Kdm6b-KO micetreated with saline or methylphenidate (MPH) in a 5-min periodfor 30 min in the open-field chamber. For each treatment, n = 8 males and 7 females per genotypes. P-value calculated using two-way ANOVA test and Sidak’s multiple comparisons test. The interactive effects of genotype and treatment on running distances: male: F3, 168 = 19.65, p < 0.0001; female: F3, 144 = 8.154, p < 0.0001. Post-hoc test: WT-saline vs. Het-saline: male: p < 0.0001; female: p = 0.0007. WT-MPH vs. Het-MPH: male: p = 0.4; female: p = 0.12. Error bars in graphs represent mean ± SEM. (C,D) Graphs represent the time course of CAR measurement in male (C) and female (D) wild-type and heterozygous Kdm6b-cKO mice treated with saline or MPH in a 60-min CAR testing period. For each treatment, n = 8 males and 7 females per genotypes. P-values calculated using a Log-rank (Mantel-Cox) test.The effects of genotype and treatment on CAR, male: Chi square = 10.34, df = 3, p = 0.016; female: Chi square = 10.58, df = 3, p = 0.014. The effects of genotype in response to treatments, WT-saline vs. Het-saline, male: p = 0.0436; female: p = 0.048; WT-MPH vs. Het-MPH, male: p = 0.13; female: p = 0.99.