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. 2022 May 4;298(6):102009. doi: 10.1016/j.jbc.2022.102009

Figure 1.

Figure 1

3hi2one-G4 activates GIRK4 homomeric channel.A, GIRK4 channel is activated by 3hi2one-G4 (30 μM) (TEVC). The asterisks indicate significant differences tested by unpaired Student's t test (∗∗p < 0.01) (data are mean ± SD, N = 5). B, representative traces of responses to the 3hi2one-G4 compound (30 μM) of GIRK4 channel. C, dose–response curves of the 3hi2one-G4 compound activation on the GIRK4 channel (EC50 = 12.74 μM) from TEVC recordings of Xenopus oocytes expressing GIRK4 channels (N = 5). D, dose–response curves of the 3h2one-G4 compound activation on the GIRK4 channel (EC50 = 5.15 μM) from patch-clamp recordings of HEK293 cells expressing GIRK4 channels (N > 5) (data are mean ± SD). E, the chemical structure of 3hi2one-G4. GIRK, G protein–sensitive inwardly rectifying potassium channel; HEK293, human embryonic kidney 293 cell; 3hi2one, 3-[2-(3,4-dimethoxyphenyl)-2-oxoethyl]-3-hydroxy-1-(1-naphthylmethyl)-1,3-dihydro-2H-indol-2-one; HK, high potassium solution ND96K; LK, low potassium solution ND96; TEVC, two-electrode voltage clamp.