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. 2022 Jun 14;12:9849. doi: 10.1038/s41598-022-14051-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Photos of experimental set-ups. (A) QIAcard FTA classic card, stapled to plain A4 paper, to be secured over a glass jar opening with an elastic band; (B) bug number experiment with 25 blood-fed 3rd/4th instar R. prolixus; (C) process to invert glass jars; (D) aggregating behaviour of 3rd/4th instar R. prolixus; (E) time point assay experiment; (F) temperature experiment with incubator set to 32 °C for 24 h; (G) darkness experiment with blackout blanket over inverted jars; (H) blood-feeding the triatomines with Hemotek feeder using equine blood; (I) appearance of fed (left) vs. unfed bugs (right) in feeding status experiment.