Figure S2.

RY785 does not affect Kv2.1 K⁺ current activation kinetics. (A) Representative K⁺ current traces during our two-pulse protocol in the presence of RY785. Overlaid traces from a representative cell during sequential steps to +40 mV from −100 mV (red traces), fitted with a single exponential function (Eq. 4, gray curves). Later traces are smaller in amplitude. Postpulse was to −60 mV in this cell. (B) Activation τ ± SD from fits as in A plotted against the corresponding trace’s peak current relative to that recorded during the first +40-mV step in RY785. Different symbols represent n = 10 cells. Stars correspond to the cell in A. Only measurements with >10% remaining current are presented, as fitted time constants became noisier at smaller current amplitudes.