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. Author manuscript; available in PMC: 2023 May 30.
Published in final edited form as: J Proteomics. 2022 Mar 21;260:104561. doi: 10.1016/j.jprot.2022.104561

Figure 2: Peptide characteristics of workflow-specific label-free phosphopeptides.

Figure 2:

Box-and-whisker plots illustrating the distribution of A) peptide length, B) mass-to-charge ratio, C) XCorr, D) GRAVY (Grand Average of Hydropathy) index, E) instability index, and F) aliphatic index. Only phosphopeptides that were exclusive to a given workflow were compared (n=4,254 for the magnetic bead strategy and n=4,124 for the spin column strategy). The center line specifies the median; box limits indicate the 25th and 75th percentiles; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles and the white dot in the box indicates the mean value.