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. 2022 May 31;9:913841. doi: 10.3389/fvets.2022.913841

Figure 5.

Figure 5

Relative mRNA levels of genes associated with lipid metabolism (A) relative mRNA levels of genes involved in the PPAR/SREBP signaling pathway in LMH cells transfected with miRNA mimics. (B) Validation of the siRNAs for silencing of HACD2, FBOX8, and TM9SF3 by RT-qPCR analysis. (C) Validation of the siRNAs for silencing of HACD2, FBOX8, and TM9SF3 by immunoblotting analysis. (D) RT-qPCR analysis of genes associated with the PPAR/SREBP signaling pathway in target-inhibited LMH cells. The results are presented as the mean and standard error of at least triplicate experiments. *P < 0.05 and **P < 0.01.