TABLE 1.
Carbon | Chemical shift (ppm) | Relative enrichmenta |
---|---|---|
1 | 14.39 | 13.3 |
2 | 23.60 | 5.6 |
3 | 33.00 | 6.8 |
4 | 30.59 | 8.8 |
5 | 30.30 | 9.6 |
6 | 26.07 | 7.5 |
7 | 30.68 | cp∗ |
8 | 19.23 | 4.8 |
9 | 35.09 | og# |
10 | 30.80 | og# |
11 | 41.25 | 4.6 |
12 | 73.00 | og# |
13 | 141.44 | 3.7 |
14 | 123.82 | 4.7 |
15 | 98.95 | 3.7 |
16 | 119.46 | 4.2 |
17 | 141.44 | 4.7 |
18 | 86.98 | 5.4 |
19 | 101.78 | 6.3 |
20 | 11.59 | 5.9 |
21 | 153.33 | 3.6 |
22 | 153.44 | 3.5 |
23 | 173.47 | 5.6 |
24 | 173.87 | 5.4 |
25 | 174.06 | og# |
26 | 180.25 | og# |
27 | 196.74 | 3.2 |
28 | 198.60 | 4 |
Calculated as α IC-XE/IC-XN, where α is determined by IC-7N/IC-7E. C-X is the carbon number, C-7 is the reference carbon, IC-XN is the intensity of the NMR signal of carbon X naturally enriched, and IC-XE is the intensity of the NMR signal of carbon X enriched in the presence of [1,2-13C2]acetate. The calculation takes into account the experimental 13C NMR conditions. cp∗, C1 pool; og#, glutamate origin.