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. 2022 May 31;13:895844. doi: 10.3389/fmicb.2022.895844

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Copyright © 2022 Jiang, Yang, Liu, Tian, Wang, Wang, Zhang, Yu, Qi, Jiang and Hsiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genomic organization of AfPV1 and SatRNA. (A) Agarose gel electrophoresis of the dsRNA segments extracted from A. flavus isolate LD-3-8 and ZD1.22-10. (B) Genome organization of dsRNA1, dsRNA2, and dsRNA3 indicated by the diagrammatic representation. (C) Detection of dsRNA1, dsRNA2, dsRNA3, and dsRNA4 by northern blot. Probe-dsRNA1, probe-dsRNA2, probe-dsRNA3, and probe-dsRNA4 were mixed or used separately to detect RNAs. (D) Secondary structure analysis of the dsRNA4 using RNAstructure 6.3 (Lu et al., 2006) showed that ribonucleotides are involved in the formation of secondary structures and predicted the presence of stem-loop structures. (E) Identity at the 5′-terminal of dsRNA1, dsRNA2, dsRNA3, and dsRNA4. Gray shading indicates that nucleotides are identical in the three segments.