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. 2022 May 31;13:895844. doi: 10.3389/fmicb.2022.895844

FIGURE 2.

FIGURE 2

Effects of AfPV1 and satellite RNA in A. flavus. (A) Paired-cultures between the donor isolate ZD1.22-10-9 (left) and the virus-free recipient isolate LD-F1 (right). Derivative isolates were obtained from the mycelial agar plugs of LD-F1. (B) Agarose gel electrophoresis of dsRNA extracted from derivative isolates (left), and RT-PCR detection for AfPV1 and satellite RNA (right). Colony morphology of isolates LD-3-8, ZD1.22-10-9, LD-F1, LD-F1-a, and LD-F1-b after culturing on CZ (C) and PDA (D) for 6 days. (E) Sporulation of isolates LD-F1, LD-F1-b, and LD-F1-a (left). The accumulation of AfPV1 in isolates LD-F1-a and LD-F1-b (right). Isolate LD-3-8 was infected with AfPV1, virus-free isolate LD-F1 was obtained from isolate LD-3-8 by single sporing, and then was labeled with a pyrithiamine resistance (ptr) gene, and isolate LD-F1-b was one of the derivative isolates, obtained by transferring AfPV1 from isolate LD-3-8 (donor) to the virus-free isolate LD-F1 (recipient). Isolate ZD1.22-10-9 was infected with AfPV1 and satellite RNA, while isolate LD-F1-a was one of the derivative isolates, obtained by transferring AfPV1 and satellite RNA from isolate ZD1.22-10-9 (donor) to the virus-free isolate LD-F1 (recipient). *P < 0.05, **P < 0.01, ***P < 0.001, by Tukey’s multiple comparison tests.