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. 2022 May 31;9:921481. doi: 10.3389/fvets.2022.921481

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Copyright © 2022 Zajac, Sangewar, Lokhandwala, Bray, Sang, McCall, Bishop, Waghela, Kumar, Kim and Mwangi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ASFV pp220 construct antigen expression. (A) Antigen expression was evaluated by immunostaining of HEK 293A cells transfected with pcDNA or infected with adenovirus encoding each pp220 construct, and (B) Western Blot, using proteins produced by transfected HEK 293A cells, probed with ASFV-specific convalescent porcine serum. Recombinant ASFV p62 antigen served as a positive control, whereas an irrelevant recombinant antigen, TMSP7, served as a negative control.