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. 2022 May 31;9:921481. doi: 10.3389/fvets.2022.921481

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Copyright © 2022 Zajac, Sangewar, Lokhandwala, Bray, Sang, McCall, Bishop, Waghela, Kumar, Kim and Mwangi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of Induced anti-pp220 antibodies: Authenticity of the antibodies elicited by the adenovirus-vectored pp220 antigens was confirmed by Immunohistochemistry. Formalin-fixed ASFV (Georgia 2007/1) infected swine spleen tissues were probed with sera that was obtained from each group two weeks post-boost: (A) Ad-pp220-ENABL; (B) Ad-pp220-ZTS-01; (C) Secondary (FITC) antibody control: secondary antibody is probed in the absence of primary sera, (D) Positive control serum: ASFV specific convalescent swine serum, and (E) Negative control serum: Ad-Luc-ENABL.