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. 2022 May 31;9:921481. doi: 10.3389/fvets.2022.921481

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Copyright © 2022 Zajac, Sangewar, Lokhandwala, Bray, Sang, McCall, Bishop, Waghela, Kumar, Kim and Mwangi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Screening of the predicted pp220 peptide pools. Five pools (Pool A-E) of predicted SLA I-binding peptides from the ASFV (Georgia 2007/1) pp220 polyprotein (Table 2) were used to stimulate (A) PBMCs or (B) splenocytes isolated from pigs immunized with the Ad-pp220-ENABL^® formulation, which were then evaluated for antigen-specific recall IFN-γ responses by EliSpot. Data is presented as Spot Forming Cells (SFC)/10⁶ PBMCs or splenocytes for each pig. Medium alone served as the negative control, and the data shown is minus media background counts.