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. Author manuscript; available in PMC: 2022 Jun 14.
Published in final edited form as: Cell Rep. 2022 May 24;39(8):110839. doi: 10.1016/j.celrep.2022.110839

Figure 5. Homogeneous conjugation allows for enhanced payload delivery to orthotopically xenografted GBM tumors for several days.

Figure 5.

(A) Study schedule for intravital imaging (male NSG mice). FITC-conjugated dextran was injected right before each imaging session to visualize the brain microvasculature.

(B–D) Intravital images of GBM12-RFP tumors treated with homogeneous Cy5.5 conjugate 8 (n = 3, B), Cys-Cy5.5 conjugate 9 (n = 4, C), and Lys-Cy5.5 conjugate 10 (n = 3, D). Representative images at each time point are shown. Scale bar: 100 μm.

(E) Normalized Cy5.5 intensity (Cy5.5 signal/GBM12-RFP signal, mean values ± SEM, n = 3 for conjugates 8 and 10; n = 4 for conjugate 9). One-way ANOVA with a Dunnett’s post hoc test (control: homogeneous conjugate 8) was used for statistical analysis.

Pre-Tx, pre-treatment.