Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 31;13:840388. doi: 10.3389/fimmu.2022.840388

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Chang, Liang, Lu, Tseng, Tsai, Tang, Sugata, Chen, Chen, Wu, Lin, Khoo and Angata

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Sialylated O-glycoproteins protect chronic lymphocytic leukemia B cells from NK cell cytotoxicity. (A) Effects of sialidase or O-sialoglycoprotein endopeptidase (OSGP-EP) treatment of JVM-3 cells on NK cell cytotoxicity. JVM-3 cells were treated with sialidase or OSGP-EP and subjected to cytotoxicity assay using an NK-92 cell line expressing Siglec-7 (NK-92MI/S7). Both treatments made JVM-3 cells more sensitive to NK cell cytotoxicity. Cytotoxicity assays were conducted in technical triplicate and repeated several times, with consistent results. Representative results are shown (*P < 0.05, **P < 0.01, and ***P < 0.001; Student’s t test). Bars represent mean ± SD of technical triplicates. (B) Effect of benzyl-2-acetamido-2-deoxy-α-d-galactopyranoside (benzyl-α-GalNAc) treatment of JVM-3 on NK cell cytotoxicity. JVM-3 cells cultured in the presence of benzyl-α-GalNAc (72 h) were more sensitive to NK cell cytotoxicity. Cytotoxicity assays were conducted in technical triplicate and repeated several times, with consistent results. Representative results are shown (*P < 0.05, Student’s t test). Bars represent mean ± SD of technical triplicates.