Skip to main content
. 2022 Jun 14;13:3426. doi: 10.1038/s41467-022-31132-7

Fig. 6. CXCL4 and TLR8 costimulation activates TBK1-IRF5 and inflammatory gene expression.

Fig. 6

a Immunoblot of IRF5 using whole-cell lysates run on nondenaturing gels. HSP90α serves as loading control. b Immunoblot of whole-cell lysates immunoprecipitated with TBK1 antibodies. c qPCR analysis of indicated mRNAs normalized relative to GAPDH mRNA in monocytes nucleofected with control or IRF5-specific siRNAs (n = 7 independent experiments). d Immunoblots of phospho-TBK1 and total TBK1 with whole-cell lysates after LPS or (CXCL4 + ORN8L) stimulation for 30 min or 3 h in the presence or absence of IKKα/β inhibitor (BMS-345541, 10 µM) or TAK1 inhibitor (Takinib, 10 µM). Data (a, b, d) are representative of three experiments and depicted as mean ± SEM (c). *p ≤ 0.05 by Wilcoxon signed-rank test, two-tailed (c). Source data are provided as a Source data file.