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. 2022 Jun 13;19:40. doi: 10.1186/s12989-022-00480-z

Fig. 5.

Fig. 5

Micrographs of leukocytes in BAL fluid from mice exposed to CuO NPs and necropsied on Days 3, 12 and 27 and control mice. Aggregated CuO NPs are visible on Day 3 inside the macrophages as well as free between the cells (thin black arrows). Recruitment of neutrophils is present on Day 3–Day 27 (thick black arrows). The appearance of macrophages (thick red arrows) changes overtime; becoming progressively more activated and enlarged from Day 3 to Day 27. Cells were stained with Protocol® HEMA 3 stain set